If there is any one secret of success, it lies in the ability to get the other person’s point of view
and see things from that person’s angle as well as from your own
Henry Ford
DSC 214 POLYMA DIFFERENTIAL SCANNING CALORIMETER AND THERMO-MICROBALANCE TG 209 F3 Tarsus® FOR THERMOGRAVIMETRIC ANALYSIS
Dear colleagues,
We are pleased to announce that the PUMS Core Facility has acquired another specialized equipment, which is available under Virtual Access Paths. Differential Scanning Calorimeter (DSC) DSC 214 Polyma and thermo-microbalance TG 209 F3 Tarsus® used for thermogravimetric analysis (TG).
The differential scanning calorimeter works on the basis of the heat-flux principle during thermal transition. It is used to determine the temperature and thermal effects of endo- and exothermic changes of various chemical reactions and phase changes. The processes that can be studied by DSC techniques are: melting, crystallization, evaporation, sublimation, phase equilibrium, glass and polymorphic transformations, formation of inclusion complexes, compound formation, dehydration, isomerization, adsorption and decomposition.
The TG 209 F3 Tarsus® micro-thermobalance is used for thermogravimetric analysis. It allows to determine the individual stages of weight loss, residual mass, temperature peaks on the mass change derivative curve (mass change rate analysis) and moisture. It is also used to analyze the composition of materials, identification of decomposition products, characteristics of raw materials as well as to determine and compare the thermal stability of materials.
The basic parameters and capabilities of the devices are available on our website tab DIFFERENTIAL SCANING CALORIMETER and THERMO-MICROBALANCE
We welcome you to cooperate.
PUMS Core Facility – We are here to help!
INFORMATION
Dear colleagues,
We would like to inform, that commmissioned research, with a completion time not longer than one week (ad hoc studies, situations associated with equipment failures), will be be accepted without sending Cooperation Forms. In the case of such orders, only an entry in the test book, available at the secretary’s office, is sufficient.
PUMS Core Facility – We are here to help!
PUMS CORE FACILITY RESUMES ACTIVITY
Dear colleagues,
We would like to inform you that on November 2, the Poznan University of Medical Sciences Core Facility resumed its scientific and research activities. We kindly ask the Principals to contact us by phone, e-mail or via the MS Teams platform in order to arrange the details of cooperation and to send the Cooperation Forms by e-mail or by PUMS internal mail.
Due to the activities of the University Coronavirus Laboratory in our Core Facility laboratories, the possibility of performing tests independently by the Principals is temporarily suspended.
The secretary’s office and Core Facility offices are located on the third floor of the Medical Biology Center in rooms 4052 and 4053.
PUMS Core Facility – We are here to help!
WE HELP TO FIGHT COVID-19
Dear colleagues,
Poznan University of Medical Sciences Core Facility has been temporarily transformed into University Coronavirus Laboratory and has joined the list of recommended diagnostic laboratories testing SARS-CoV-2 virus.
All information regarding the laboratory’s activities is available on the links below:
http://www.uczelnia.ump.edu.pl/covid/
https://www.ump.edu.pl/aktualnosci/pomagamy-w-walce-z-covid-19
PUMS Core Facility – We are here to help!
NEW REGULATIONS
Dear colleagues,
With the introduction of the new statue of the Poznan University of Medical Sciences, some changes also apply to the functioning of our Core Facility. Please follow the link below for more information (http://www.uca.ump.edu.pl/en/documents/).
Your cooperation is welcome!
PUMS Core Facility – We are here to help!
ANIMAL HOUSE IS NOW OPEN
Dear colleagues,
Animal house of the Poznan University of Medical Sciences located at our Core Facility was registered and officially commissioned in January 2018. For the past 18 months of its functioning, we were completing and improving the qualifications of our unit’s staff. Until now, animals have only visited the Laboratory once, during a short two-week experiment. Today (6.08.2019) we are pleased to announce the first long-term experiment with laboratory animals (rats). The person responsible for this project, entrusting us with the animals for the next several months is Professor Ewa Florek from Laboratory of Environmental Research at the Department of Toxicology.
Taking this opportunity, we would like to encourage people interested in conducting experiments at the animal house, to read the principles of cooperation in this area, including Regulations of the Laboratory of Experimental Animals and work instructions in front of (conventional animals) and behind the barrier zone (SPF animals).
These regulations and instructions are available in the Documents tab on the unit’s website (http://uca.ump.edu.pl/en/documents/).
Following the link below you will find other information on the functioning of the animal house (http://uca.ump.edu.pl/en/laboratory-of-experimental-animals/). If you have any questions, please contact the Head of the Laboratory, Emilia Cicha, Vet. (emiliacicha@ump.edu.pl or +48 61 854 77 13).
We are convinced that, just like in the past two years you entrusted us in conducting exactly 50 projects, the Laboratory of Experimental Animals also will gain your acceptance.
Your cooperation is welcome!
PUMS Core Facility – We are here to help!
OPEN DAY
Dear colleagues,
Facing many questions about the scientific research at the PUMS Core Facility we kindly invite all interested employees of the Medical University to the OPEN DAY, which will be held on June, 10th at 10:00 AM.
People interested in visiting specialized laboratories of the PUMS Core Facility and the animal house, as well as getting acquainted with our research offer, are asked to contact the Head of the Unit, Professor Krzysztof Książek (kksiazek@ump.edu.pl).
The number of places is limited.
PUMS Core Facility – We are here to help!
DROPLET DIGITAL PCR QX200
We are pleased to announce that our unit has purchased a third-generation PCR system – Bio-Rad’s QX200 Droplet Digital PCR (ddPCR). This technology enables high-throughput digital PCR in a manner that uses lower sample and reagent volumes and reduces overall cost compared with other methods while maintaining sensitivity and precision.
ddPCR is a method based on water-oil emulsion droplet technology. A sample is fractionated into 20,000 droplets and PCR amplification of the template molecules occurs in each individual droplet. ddPCR technology uses reagents and workflows similar to those used for most standard TaqMan probe-based assays. The massive sample partitioning is a key aspect of the ddPCR technique
Moreover, this technique provides an absolute count of target DNA copies per input sample, without the need for running standard curves or reference genes. The ddPCR system can be used to detect rare DNA target copies with unmatched sensitivity; determine copy number variation with unrevealed accuracy and measure gene expression levels with exquisite precision. It is possible to work with FAM, VIC and HEX fluorescent dyes, as well as with TaqMan probes and Eva Green dye.
It must be noted, that the system allows to determine changes in the level of gene expression of 10% and less (which cannot be performed with the second generation qPCR), and to detect a mutation at the level of 0.001% (qPCR is difficult to detect at the level of 1%). Detailed information on the applications of the QX200 ddPCR system can be found on the website of the manufacturer: (http://www.bio-rad.com/en-pl/product/qx200-droplet-digital-pcr-system).
www.bio-rad.com
DEPARTMENT OF GENETIC ENGINEERING
We are happy to announce that by the decision of the Minister of the Environment of July 2, 2018 PUMS Core facility has received the status of the Genetic Engineering Department category II. This gives permission to work with genetically modified microorganisms (GMMs), classified as risk category II (http://prawo.sejm.gov.pl/isap.nsf/download.xsp/WDU20160000624/O/D20160624.pdf).
This license enables the implementation of scientific projects in the field of broadly understood molecular biology, for which it is necessary to use viral and / or bacterial vectors (in accordance with the granted category). In order to perform experiments with GMMs, it is required to obtain individual consent from the Ministry of the Environment.
We kindly invite you to cooperation!
GUAVA FLOW CYTOMETER
We are glad to announce that our unit has acquired a high-class GUAVA EasyCyte 6HT-2L flow cytometer. The instrument is equipped with 2 most commonly used lasers (488 nm and 642 nm with 40 mW power) and four reading channels (green – 525/30 nm; yellow – 583/26 nm, red 1 – 690/50 nm and red 2 – 661 / 19 nm). A unique feature of the device is the ability to work in a traditional test tube system or in a plate system, allowing the simultaneous collection and analysis of 96 samples! Another, undeniable advantage of the GUAVA cytometer is the ability to perform valuable measurements using only 2,000 cells (with the optimal, recommended number of 20,000).
We kindly invite you to cooperation!
BIOBANK
We are pleased to announce that our Core Facility has joined the Polish Biobank Network, i.e. an association of national scientific and research units specializing in biological material banking. The network belongs to the European Research Consortium, which provides controlled access to biobanking resources for academia and industry. In the next two years our unit will have the Observer status, which, apart from developing the unit’s offer in the field of biological material banking, will also enable the development and implementation of the highest standards of handling biological material. We strongly encourage all researchers of the Medical University, including representatives of clinical and basic sciences, to cooperate with us in the field of biological material banking and to work together to develop our knowledge in and experience in this field.
PUMS Core Facility – We are here to help!
VIRTUAL ACCESS
Dear Medical University Researchers,
We encourage you to place orders for research using the equipment available under Virtual Access Paths (http://www.uca.ump.edu.pl/en/virtual-access-paths/).
Ultracentrifuge Thermo Sorvall MX120 +
Dear colleagues,
We are pleased to announce that our unit has purchased the Thermo Sorvall MX120 + ultracentrifuge. This apparatus is equipped with a fixed-angle rotor, enabling samples to be centrifuged at a speed of up to 80,000 revolutions per minute (414,600 x g). We encourage you to use this ultramodern device in planning new experiments.
PUMS Core Facility website
We are pleased to present the new version of our website. We believe that the information on this webpage will be useful to all the researchers, and above all, that it will help to establish numerous and fruitful cooperation between the units of our University and the Core Facility.
PUMS Core Facility – We are here to help!